Recombinant human HSP27 protein
Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38) . Recombinant human HSP27 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Heat shock protein beta-1, CMT2F, HMN2B, Hsp25, Heat shock protein beta-1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
24.9 kDa (225aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Friedman MJ., et al. (2009) J Biol Chem. 284 (41) :27944-51. ; ; Brunet Simioni M., et al. (2009) Oncogene. 28 (37) :3332-44.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_001531
Uniprot Accession Number
P04792
Species
Human
AA Sequence
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