Recombinant human Glutathione S-transferase alpha 1/GSTA1 protein

GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 35,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Product Name Alternative

Glutathione S transferase A1, Glutathione S-transferase alpha 1, GST epsilon, GST2, GSTA1, GSTA1-1, GTH1, HA subunit 1

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 35,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Molecular Weight

25.6 kDa (222aa) confirmed by MALDI-TOF

Additionnal Information

Glutathione S-transferase a1, GSTA1, Glutathione S-transferase alpha 1, GST2, GSTA1-1, GTH1, Glutathione S-transferase alpha 1 Glutathione S transferase A1, GST epsilon, GSTA1, GSTA1 1, HA subunit 1., ATGP0443-10 µg, ATGP0443-20 µg, ATGP0443-50 µg, ATGP0443-100 µg, ATGP0443-250 µg, ATGP0443-500 µg, ATGP0443-1 mg, ATGP0443-10, ATGP0443-20, ATGP0443-50, ATGP0443-100, ATGP0443-250, ATGP0443-500, ATGP0443-1

References & Citations

Rossi D., et al. (2009) Leukemia. 23 (6) :1118-26. ; ; Hou L., et al. (2007) J Biol Chem. 282 (32) :23264-74.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_665683

Uniprot Accession Number

P08263

Species

Human

AA Sequence

MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF