What is the catalog number for Recombinant human FEN1 protein?

The catalog number for Recombinant human FEN1 protein is ATGP0442-100.

What sizes are available for Recombinant human FEN1 protein?

Recombinant human FEN1 protein is available in the following size(s): 100 µg.

Is Recombinant human FEN1 protein in stock?

Recombinant human FEN1 protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human FEN1 protein

FEN-1, also known as Flap structure-specific endonuclease 1, removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication. Recombinant human FEN-1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Flap structure-specific endonuclease 1, FEN-1, MF1, RAD2, Flap structure-specific endonuclease 1 DNase IV, Flap endonuclease 1, Flap structure specific endonuclease 1, hFEN1, Maturation factor 1

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

42.5 kDa (380aa) confirmed by MALDI-TOF

Additionnal Information

FEN1, Flap structure-specific endonuclease 1, FEN-1, MF1, RAD2, Flap structure-specific endonuclease 1 DNase IV, Flap endonuclease 1, Flap structure specific endonuclease 1, hFEN1, Maturation factor 1, ATGP0442-10 µg, ATGP0442-20 µg, ATGP0442-50 µg, ATGP0442-100 µg, ATGP0442-250 µg, ATGP0442-500 µg, ATGP0442-1 mg, ATGP0442-10, ATGP0442-20, ATGP0442-50, ATGP0442-100, ATGP0442-250, ATGP0442-500, ATGP0442-1

References & Citations

Hakoyama T., et al. (2009) Nature. 462 (7272) :514-7. ; ; Lal A., et al. (2009) Mol Cell. 35 (5) :610-25.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_004102.1

Uniprot Accession Number

P39748

Species

Human

AA Sequence

MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK