Recombinant human ACADS protein

ACADS (Acyl-Coenzyme A dehydrogenase), also known as SCAD or ACAD3, is a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations of ACADS have been associated with fatty acid oxidation defects and metabolic diseases such as short-chain acyl-CoA dehydrogenase deficiency (SCAD deficiency) . Recombinant human ACADS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Acyl-Coenzyme A dehydrogenase, ACAD3, SCAD, Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, short chain, Acyl-Coenzyme A dehydrogenase, short chain, AI196007, Bcd-1, Bcd1, Butyryl CoA dehydrogenase, EC 1.3.99.2, Short chain acyl CoA dehydrogenase, unsaturated acyl CoA reductase, Short-chain specific acyl-CoA dehydrogenase, mitochondrial.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

44.0 kDa (409aa) confirmed by MALDI-TOF

Additionnal Information

ACADS, Acyl-Coenzyme A dehydrogenase, ACAD3, SCAD, Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, short chain, Acyl-Coenzyme A dehydrogenase, short chain, AI196007, Bcd-1, Bcd1, Butyryl CoA dehydrogenase, EC 1.3.99.2, Short chain acyl CoA dehydrogenase, unsaturated acyl CoA reductase, Short-chain specific acyl-CoA dehydrogenase, mitochondrial., ATGP0441-10 µg, ATGP0441-20 µg, ATGP0441-50 µg, ATGP0441-100 µg, ATGP0441-250 µg, ATGP0441-500 µg, ATGP0441-1 mg, ATGP0441-10, ATGP0441-20, ATGP0441-50, ATGP0441-100, ATGP0441-250, ATGP0441-500, ATGP0441-1

References & Citations

McAndrew RP., et al. (2008) J Biol Chem. 283 (14) :9435-43. ; ; Tafti M., et al. (2003) Nat Genet. 34 (3) :320-5.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 0.1 M Nacl, and 20% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

AAH25963

Uniprot Accession Number

P16219

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MLHTIYQSVE LPETHQMLLQ TCRDFAEKEL FPIAAQVDKE HLFPAAQVKK MGGLGLLAMD VPEELGGAGL DYLAYAIAME EISRGCASTG VIMSVNNSLY LGPILKFGSK EQKQAWVTPF TSGDKIGCFA LSEPGNGSDA GAASTTARAE GDSWVLNGTK AWITNAWEAS AAVVFASTDR ALQNKSISAF LVPMPTPGLT LGKKEDKLGI RGSSTANLIF EDCRIPKDSI LGEPGMGFKI AMQTLDMGRI GIASQALGIA QTALDCAVNY AENRMAFGAP LTKLQVIQFK LADMALALES ARLLTWRAAM LKDNKKPFIK EAAMAKLAAS EAATAISHQA IQILGGMGYV TEMPAERHYR DARITEIYEG TSEIQRLVIA GHLLRSYRS