Recombinant human BAG3 protein

BAG3 was reported initially as a protein-refolding cochaperone of the bcl2 binding protein BAG family and as upregulated in response to persistent stress of cellular calcium balance dysregulation. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. Also, it has been shown to diminish stress-induced apoptosis. Recombinant human BAG3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

BAG family molecular chaperone regulator 3, BIS, CAIR-1, BAG family molecular chaperone regulator 3 BAG 3, Bcl 2 binding protein, BCL2 associated athanogene 3, BCL2 binding athanogene 3, Docking protein CAIR 1.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

63.7 kDa (595aa)

Additionnal Information

BAG3, BAG family molecular chaperone regulator 3, BIS, CAIR-1, BAG family molecular chaperone regulator 3 BAG 3, Bcl 2 binding protein, BCL2 associated athanogene 3, BCL2 binding athanogene 3, Docking protein CAIR 1., ATGP0440-10 µg, ATGP0440-20 µg, ATGP0440-50 µg, ATGP0440-100 µg, ATGP0440-250 µg, ATGP0440-500 µg, ATGP0440-1 mg, ATGP0440-10, ATGP0440-20, ATGP0440-50, ATGP0440-100, ATGP0440-250, ATGP0440-500, ATGP0440-1

References & Citations

Virador VM., et al. (2009) PLos One. 4 (4) :e5136. ; ; Kyratsous CA., et al. (2008) Proc Natl Acad Sci u S A. 105 (52) :20912-7.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA, 0.1mM PMSF, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_004272.2

Uniprot Accession Number

O95817

Species

Human

AA Sequence

MSAATHSPMM QVASGNGDRD PLPPGWEIKI DPQTGWPFFV DHNSRTTTWN DPRVPSEGPK ETPSSANGPS REGSRLPPAR EGHPVYPQLR PGYIPIPVLH EGAENRQVHP FHVYPQPGMQ RFRTEAAAAA PQRSQSPLRG MPETTQPDKQ CGQVAAAAAA QPPASHGPER SQSPAASDCS SSSSSASLPS SGRSSLGSHQ LPRGYISIPV IHEQNVTRPA AQPSFHQAQK THYPAQQGEY QTHQPVYHKI QGDDWEPRPL RAASPFRSSV QGASSREGSP ARSSTPLHSP SPIRVHTVVD RPQQPMTHRE TAPVSQPENK PESKPGPVGP ELPPGHIPIQ VIRKEVDSKP VSQKPPPPSE KVEVKVPPAP VPCPPPSPGP SAVPSSPKSV ATEERAAPST APAEATPPKP GEAEAPPKHP GVLKVEAILE KVQGLEQAVD NFEGKKTDKK YLMIEEYLTK ELLALDSVDP EGRADVRQAR RDGVRKVQTI LEKLEQKAID VPGQVQVYEL QPSNLEADQP LQAIMEMGAV AADKGKKNAG NAEDPHTETQ QPEATAAATS NPSSMTDTPG NPAAP