Recombinant human BAG3 protein
BAG3 was reported initially as a protein-refolding cochaperone of the bcl2 binding protein BAG family and as upregulated in response to persistent stress of cellular calcium balance dysregulation. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. Also, it has been shown to diminish stress-induced apoptosis. Recombinant human BAG3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
63.7 kDa (595aa)
Additionnal Information
References & Citations
Virador VM., et al. (2009) PLos One. 4 (4) :e5136. ; ; Kyratsous CA., et al. (2008) Proc Natl Acad Sci u S A. 105 (52) :20912-7.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA, 0.1mM PMSF, 10% glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_004272.2
Uniprot Accession Number
O95817
Species
Human
AA Sequence
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