Recombinant human CapG protein

Macrophage-capping protein, also known as CAPG, belongs to the gelsolin/villin family of actin-regulatory proteins. CAPG is a calcium-sensitive DNA-binding protein that plays a role in macrophage function. It is expressed in macrophages and macrophage-like cells and can localize both to the nucleus and the cytoplasm. This protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. Recombinant human CAPG was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Macrophage-capping protein, MCP, AFCP, Macrophage-capping protein Actin capping protein GCAP39, Actin regulatory protein CAP G, CAPG, Capping protein (actin filament) gelsolin like, Capping protein gelsolin like, gCap39, Gelsolin like capping protein, Macrophage capping protein, mbh1, Myc basic motif homolog 1.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

38.5 kDa (348aa) confirmed by MALDI-TOF

Additionnal Information

CapG, Macrophage-capping protein, MCP, AFCP, Macrophage-capping protein Actin capping protein GCAP39, Actin regulatory protein CAP G, CAPG, Capping protein (actin filament) gelsolin like, Capping protein gelsolin like, gCap39, Gelsolin like capping protein, Macrophage capping protein, mbh1, Myc basic motif homolog 1., ATGP0439-10 µg, ATGP0439-20 µg, ATGP0439-50 µg, ATGP0439-100 µg, ATGP0439-250 µg, ATGP0439-500 µg, ATGP0439-1 mg, ATGP0439-10, ATGP0439-20, ATGP0439-50, ATGP0439-100, ATGP0439-250, ATGP0439-500, ATGP0439-1

References & Citations

Dabiri G A., et al. (1992) J Biol Chem. 267:16545-16552.; ; Pellieux C., et al. (2003) J Biol Chem. 278 (3) :29136-44.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

AAH00728

Uniprot Accession Number

P40121

Species

Human

AA Sequence

MYTAIPQSGS PFPGSVQDPG LHVWRVEKLK PVPVAQENQG VFFSGDSYLV LHNGPEEVSH LHLWIGQQSS RDEQGACAVL AVHLNTLLGE RPVQHREVQG NESDLFMSYF PRGLKYQEGG VESAFHKTST GAPAAIKKLY QVKGKKNIRA TERALNWDSF NTGDCFILDL GQNIFAWCGG KSNILERNKA RDLALAIRDS ERQGKAQVEI VTDGEEPAEM IQVLGPKPAL KEGNPEEDLT ADKANAQAAA LYKVSDATGQ MNLTKVADSS PFALELLISD DCFVLDNGLC GKIYIWKGRK ANEKERQAAL QVAEGFISRM QYAPNTQVEI LPQGRESPIF KQFFKDWK