Recombinant human Ketohexokinase protein

Ketohexokinase is an enzyme that catalyzes the phosphorylation of fructose to produce fructose-1-phosphate, leading to consumption of ATP, formation of AMP. This protein initiates first step in the metabolism of dietary fructose and is an important regulator of hepatic glucose metabolism. It is highly found in liver, renal cortex, and small intestine. Its deficiency causes the benign hereditary metabolic disorder essential fructosuria, leading to fructose being excreted in the urine. Recombinant human Ketohexokinase was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

KHK, Hepatic fructokinase, EC 2.7.1.3, Hepatic fructokinase, ketohexokinase, Ketohexokinase isoform a

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.7 kDa (298aa) confirmed by MALDI-TOF

Additionnal Information

Ketohexokinase, KHK, Hepatic fructokinase, EC 2.7.1.3, Hepatic fructokinase, ketohexokinase, Ketohexokinase isoform a, ATGP0437-10 µg, ATGP0437-20 µg, ATGP0437-50 µg, ATGP0437-100 µg, ATGP0437-250 µg, ATGP0437-500 µg, ATGP0437-1 mg, ATGP0437-10, ATGP0437-20, ATGP0437-50, ATGP0437-100, ATGP0437-250, ATGP0437-500, ATGP0437-1

References & Citations

Bonthron DT., et al. (2009) J Histochem Cytochem. 57 (8) :763-74.; ; Schermerhorn T., et al. (2009) Res Vet Sci. 87 (1) :115-7. ;

Other References

Wuest M, et al. Radiopharmacological evaluation of 6-deoxy-6-[18F]fluoro-D-fructose as a radiotracer for PET imaging of GLUT5 in breast cancer. (Nucl Med Biol. 2011) {https://pubmed.ncbi.nlm.nih.gov/21531283/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

AAH06233

Uniprot Accession Number

P50053

Species

Human

AA Sequence

MEEKQILCVG LVVLDVISLV DKYPKEDSEI RCLSQRWQRG GNASNSCTIL SLLGAPCAFM GSMAPGHVAD FVLDDLRRYS VDLRYTVFQT TGSVPIATVI INEASGSRTI LYYDRSLPDV SATDFEKVDL TQFKWIHIEG RNASEQVKML QRIDAHNTRQ PPEQKIRVSV EVEKPREELF QLFGYGDVVF VSKDVAKHLG FQSAEEALRG LYGRVRKGAV LVCAWAEEGA DALGPDGKLL HSDAFPPPRV VDTLGAGDTF NASVIFSLSQ GRSVQEALRF GCQVAGKKCG LQGFDGIV