Recombinant human 14-3-3 sigma protein

14-3-3 sigma, also known as Stratifin (SFN), belong to the 14-3-3 family. The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 sigma was identified as an epithelial cell marker and appeared to function as a tumor suppressor whose expression can be down regulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Recombinant human 14-3-3 sigma was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Stratifin, Epithelial cell marker protein 1, HME1, YWHAS, SFN, 14-3-3 sigma

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

27.7 kDa (248aa) confirmed by MALDI-TOF

Additionnal Information

1433 sigma, stratifin, Epithelial cell marker protein 1, HME1, YWHAS, SFN, 14-3-3 sigma, ATGP0435-10 µg, ATGP0435-20 µg, ATGP0435-50 µg, ATGP0435-100 µg, ATGP0435-250 µg, ATGP0435-500 µg, ATGP0435-1 mg, ATGP0435-10, ATGP0435-20, ATGP0435-50, ATGP0435-100, ATGP0435-250, ATGP0435-500, ATGP0435-1

References & Citations

Benzinger A., et al. (2005) Mol Cell Proteomics. 4 (6) : 785-95.; ; Wilker E W., et al. (2005) J Biol Chem. 280:18891-18898.;

Other References

Arakawa N, et al. Serum stratifin and presepsin as candidate biomarkers for early detection of COVID-19 disease progression. (J Pharmacol Sci. 2022) {https://pubmed.ncbi.nlm.nih.gov/35926945/}; ; Arakawa N, et al. Stratifin as a novel diagnostic biomarker in serum for diffuse alveolar damage. (Nat Commun. 2022) {https://pubmed.ncbi.nlm.nih.gov/36195613/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl, 10% glycerol

Scientific Category

Apoptosis

NCBI Accession Number

NP_006133

Uniprot Accession Number

P31947

Species

Human

AA Sequence

MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS