Recombinant human 14-3-3 sigma protein
14-3-3 sigma, also known as Stratifin (SFN), belong to the 14-3-3 family. The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 sigma was identified as an epithelial cell marker and appeared to function as a tumor suppressor whose expression can be down regulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Recombinant human 14-3-3 sigma was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Stratifin, Epithelial cell marker protein 1, HME1, YWHAS, SFN, 14-3-3 sigma
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
27.7 kDa (248aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Benzinger A., et al. (2005) Mol Cell Proteomics. 4 (6) : 785-95.; ; Wilker E W., et al. (2005) J Biol Chem. 280:18891-18898.;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl, 10% glycerol
Scientific Category
Apoptosis
NCBI Accession Number
NP_006133
Uniprot Accession Number
P31947
Species
Human
AA Sequence
Frequently Asked Questions
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