Recombinant human Aldo-keto reductase 1A1/AKR1A1 protein

Alcohol dehydrogenase [NADP+] (AKR1A1) is a member of the aldo/keto reductase superfamily and catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. This protein is closely related (65% identity) to aldose reductase, an enzyme involved in the pathogenesis of some diabetic and galactosemic complications. It plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAuN) . Recombinant human Alcohol dehydrogenase was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Dihydrodiol dehydrogenase 3, DD3, ARM, ALR, ALDR1, aldo-keto reductase family 1 member A1 (aldehyde reductase), Aldehyde reductase, Alcohol dehydrogenase [NADP+], Alcohol dehydrogenase, AKR1A1

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.5 kDa (325aa) confirmed by MALDI-TOF

Additionnal Information

Dihydrodiol dehydrogenase 3, DD3, ARM, ALR, ALDR1, aldo-keto reductase family 1 member A1 (aldehyde reductase), Aldehyde reductase, Alcohol dehydrogenase [NADP+], Alcohol dehydrogenase, AKR1A1, ATGP0433-10 µg, ATGP0433-20 µg, ATGP0433-50 µg, ATGP0433-100 µg, ATGP0433-250 µg, ATGP0433-500 µg, ATGP0433-1 mg, ATGP0433-010, ATGP0433-020, ATGP0433-050, ATGP0433-100, ATGP0433-250, ATGP0433-500, ATGP0433-01M

References & Citations

Bohren KM., et al. (1989) J Biol Chem. 264 (16) : 9547-51.; ; Vander Jaqt DL., et al. (1990) J Biol Chem. 265 (19) :10912-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl, 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_006057

Uniprot Accession Number

P14550

Species

Human

AA Sequence

MAASCVLLHT GQKMPLIGLG TWKSEPGQVK AAVKYALSVG YRHIDCAAIY GNEPEIGEAL KEDVGPGKAV PREELFVTSK LWNTKHHPED VEPALRKTLA DLQLEYLDLY LMHWPYAFER GDNPFPKNAD GTICYDSTHY KETWKALEAL VAKGLVQALG LSNFNSRQID DILSVASVRP AVLQVECHPY LAQNELIAHC QARGLEVTAY SPLGSSDRAW RDPDEPVLLE EPVVLALAEKYGRSPAQILL RWQVQRKVIC IPKSITPSRI LQNIKVFDFT FSPEEMKQLN ALNKNWRYIV PMLTVDGKRV PRDAGHPLYP FNDPY