Recombinant human Cyclophilin G/PPIG protein

Peptidyl-prolyl cis-trans isomerase G (PPIG), also known as Cyclophilin G, is a member of peptidyl-prolyl cis-trans isomerase family (PPIases) . This protein catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and is implicated in the folding, transport, and assembly of proteins. It is localized to the nuclear speckles, a nuclear compartment rich in splicing factors, and interacts with the splicing factors SC35 and pinin. Cyclophilin G also may play an important role in the regulation of pre-mRNA splicing. Recombinant human Cyclophilin G, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 600 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

Peptidyl-prolyl cis-trans isomerase G, PPIase G, Rotamase G, Cyclophilin G, CASP10, PPIG, Peptidyl-prolyl cis-trans isomerase G, peptidylprolyl isomerase G (cyclophilin G), CARS-Cyp, SRCyp, SCAF10, SR-related CTD-associated factor 10

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 600 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

21.6 kDa (195aa) confirmed by MALDI-TOF

Additionnal Information

Cyclophilin G, PPIG, Peptidyl-prolyl cis-trans isomerase G, PPIase G, Rotamase G, Cyclophilin G, CASP10, PPIG, Peptidyl-prolyl cis-trans isomerase G, peptidylprolyl isomerase G (cyclophilin G), CARS-Cyp, SRCyp, SCAF10, SR-related CTD-associated factor 10, ATGP0431-10 µg, ATGP0431-20 µg, ATGP0431-50 µg, ATGP0431-100 µg, ATGP0431-250 µg, ATGP0431-500 µg, ATGP0431-1 mg, ATGP0431-10, ATGP0431-20, ATGP0431-50, ATGP0431-100, ATGP0431-250, ATGP0431-500, ATGP0431-1

References & Citations

Modjtahedi N., et al. (2004) . J Biol Chem. 279 (21) :22322-30 ; ; Ouyang P., et al. (2004) . Biochem Biophys Re Commun. 321 (3) : 638-47 ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

AAH01555

Uniprot Accession Number

Q13427

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGIKVQRPRC FFDIAINNQP AGRVVFELFS DVCPKTCENF RCLCTGEKGT GKSTQKPLHY KSCLFHRVVK DFMVQGGDFS EGNGRGGESI YGGFFEDESF AVKHNKEFLL SMANRGKDTN GSQFFITTKP TPHLDGHHVV FGQVISGQEV VREIENQKTD AASKPFAEVR ILSCG