Recombinant human PKM protein
PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. PKM2 interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis. It is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. Recombinant human PKM2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Specifications
Specific activity > 25,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C.
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity: > 25,000pmol/min/ug. One unit will convert 1.0pmole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C.
Molecular Weight
60.1 kDa (551aa)
Additionnal Information
References & Citations
Zwerschke W., et al. (2009) . Exp Cell Res. 315 (16) :2765-74; ; Mazurek S., et al. (2007) . Ernst Schering Found Symp Proc. (4) :99-124.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_002645.3
Uniprot Accession Number
P14618
Species
Human
AA Sequence
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