Recombinant human Carbonic Anhydrase 1/CA1 protein
Carbonic anhydrase 1 (CA1) is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O <-> HCO3- + H+) . This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation. Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Carbonic anhydrase I, CA-I, Carbonate dehydratase I, Carbonic anhydrase B, CAB, Cyanamide hydratase CA1, Car1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
31.0 kDa (281aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nogradi A., et al. (1998) . Am J Pathol. 153:1-4; ; Ferry JG., et al. (1999) . Proc Natl Acad Sci u S A. 96 (26) :15184-9.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_001122301
Uniprot Accession Number
P00915
Species
Human
AA Sequence
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