Recombinant human Carbonic Anhydrase 1/CA1 protein

Carbonic anhydrase 1 (CA1) is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O <-> HCO3- + H+) . This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation. Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Carbonic anhydrase I, CA-I, Carbonate dehydratase I, Carbonic anhydrase B, CAB, Cyanamide hydratase CA1, Car1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

31.0 kDa (281aa) confirmed by MALDI-TOF

Additionnal Information

Carbonic Anhydrase I, CA1, CA1, CA-I, CAB, JW0122, CA 1, CA I, CAI, CAN, Car 1, Car1, Carbonate dehydratase I, Carbonic anhydrase 1, Carbonic anhydrase B, Carbonic dehydratase, ECK0125, yadF, ATGP0427-10 µg, ATGP0427-20 µg, ATGP0427-50 µg, ATGP0427-100 µg, ATGP0427-250 µg, ATGP0427-500 µg, ATGP0427-1 mg, ATGP0427-10, ATGP0427-20, ATGP0427-50, ATGP0427-100, ATGP0427-250, ATGP0427-500, ATGP0427-1

References & Citations

Nogradi A., et al. (1998) . Am J Pathol. 153:1-4; ; Ferry JG., et al. (1999) . Proc Natl Acad Sci u S A. 96 (26) :15184-9.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_001122301

Uniprot Accession Number

P00915

Species

Human

AA Sequence

MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F