Recombinant human GAMT protein

Guanidinoacetate N-methyltransferase isoform a, also known as GAMT is a methyltansferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl domor. This enzyme participates in the two-step synthesis of the compound creatine from the protein building blocks glycine, arginine, and methionine. It is involved in providing energy for muscle contraction, and is also important in nervous system functioning. Also GAMT is active in the liver, pancreas, and kidneys. Recombinant human GAMT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Guanidinoacetate N-methyltransferase isoform a, PIG2, TP53I2, Guanidinoacetate N-methyltransferase isoform a Guanidinoacetate N methyltransferase.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

28.4 kDa (256aa) confirmed by MALDI-TOF

Additionnal Information

GAMT, Guanidinoacetate N-methyltransferase isoform a, PIG2, TP53I2, Guanidinoacetate N-methyltransferase isoform a Guanidinoacetate N methyltransferase., ATGP0424-10 µg, ATGP0424-20 µg, ATGP0424-50 µg, ATGP0424-100 µg, ATGP0424-250 µg, ATGP0424-500 µg, ATGP0424-1 mg, ATGP0424-10, ATGP0424-20, ATGP0424-50, ATGP0424-100, ATGP0424-250, ATGP0424-500, ATGP0424-1

References & Citations

Almeida LS., et al. (2007) Mol Genet Metab. 91 (1) :1-6. ; ; Jenne DE., et al. (1997) Biochem Biophys Res Commun. 238 (3) :723-7. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000147

Uniprot Accession Number

Q14353

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSAPSATPIF APGENCSPAW GAAPAAYDAA DTHLRILGKP VMERWETPYM HALAAAASSK GGRVLEVGFG MAIAASKVQE APIDEHWIIE CNDGVFQRLR DWAPRQTHKV IPLKGLWEDV APTLPDGHFD GILYDTYPLS EETWHTHQFN FIKNHAFRLL KPGGVLTYCN LTSWGELMKS KYSDITIMFE ETQVPALLEA GFRRENIRTE VMALVPPADC RYYAFPQMIT PLVTKG