Recombinant human GAMT protein
Guanidinoacetate N-methyltransferase isoform a, also known as GAMT is a methyltansferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl domor. This enzyme participates in the two-step synthesis of the compound creatine from the protein building blocks glycine, arginine, and methionine. It is involved in providing energy for muscle contraction, and is also important in nervous system functioning. Also GAMT is active in the liver, pancreas, and kidneys. Recombinant human GAMT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Guanidinoacetate N-methyltransferase isoform a, PIG2, TP53I2, Guanidinoacetate N-methyltransferase isoform a Guanidinoacetate N methyltransferase.
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
28.4 kDa (256aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Almeida LS., et al. (2007) Mol Genet Metab. 91 (1) :1-6. ; ; Jenne DE., et al. (1997) Biochem Biophys Res Commun. 238 (3) :723-7. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_000147
Uniprot Accession Number
Q14353
Species
Human
AA Sequence
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