Recombinant human Rhodanese/TST protein

Thiosulfate sulfurtransferase (TST), also known as Rhodanese, is a mitochondrial enzyme that involved in cyanide detoxification and the modification of sulfur-containing enzymes. This protein contains two highly conservative domains, known as rhodanese homology domains. In mammals, most cyanide is converted to thiocyanate by this enzyme. TST also has weak mercaptopyruvate sulfurtransferase activity. Recombinant TST protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

RDS, TST, Rhodanese, Thiosulfate Sulfurtransferase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

35.5 kDa (317aa) confirmed by MALDI-TOF

Additionnal Information

Thiosulfate sulfurtransferase, RDS, TST, Rhodanese, Thiosulfate Sulfurtransferase, ATGP0423-10 µg, ATGP0423-20 µg, ATGP0423-50 µg, ATGP0423-100 µg, ATGP0423-250 µg, ATGP0423-500 µg, ATGP0423-1 mg, ATGP0423-10, ATGP0423-20, ATGP0423-50, ATGP0423-100, ATGP0423-250, ATGP0423-500, ATGP0423-1

References & Citations

Pallini R., et al. (1991) Biochem Biophys Res Commun. 180 (2) :887-93. ; ; Aita N., et al. (1997) Biochem Biophys Res Commun. 231 (1) :56-60.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_003303.2

Uniprot Accession Number

Q16762

Species

Human

AA Sequence

MVHQVLYRAL VSTKWLAESI RTGKLGPGLR VLDASWYSPG TREARKEYLE RHVPGASFFD IEECRDTASP YEMMLPSEAG FAEYVGRLGI SNHTHVVVYD GEHLGSFYAP RVWWMFRVFG HRTVSVLNGG FRNWLKEGHP VTSEPSRPEP AVFKATLDRS LLKTYEQVLE NLESKRFQLV DSRSQGRFLG TEPEPDAVGL DSGHIRGAVN MPFMDFLTED GFEKGPEELR ALFQTKKVDL SQPLIATCRK GVTACHVALA AYLCGKPDVA VYDGSWSEWF RRAPPESRVS QGKSEKA