Recombinant human HSPA8/HSC71 protein

HSC70, also known as heat shock 70 kDa protein 8, belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This protein is a heat-shock cognate protein. HSC70 binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Recombinant HSC70 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Heat shock 70 kDa protein 8, HSPA8, HSC54, HSC71, HSP71, HSP73, HSPA10, LAP1, NIP71, Heat shock 70 kDa protein 8, heat shock 70kDa protein 8, HSC70

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

73.1 kDa (666aa)

Additionnal Information

HSPA8, HSC71, Heat shock 70 kDa protein 8, HSPA8, HSC54, HSC71, HSP71, HSP73, HSPA10, LAP1, NIP71, Heat shock 70 kDa protein 8, heat shock 70kDa protein 8, HSC70, ATGP0415-10 µg, ATGP0415-20 µg, ATGP0415-50 µg, ATGP0415-100 µg, ATGP0415-250 µg, ATGP0415-500 µg, ATGP0415-1 mg, ATGP0415-10, ATGP0415-20, ATGP0415-50, ATGP0415-100, ATGP0415-250, ATGP0415-500, ATGP0415-1

References & Citations

Tsukahara F., et al. (2000) Mol Pharmacol. 58 (6) :1257-63. ; ; Dworniczak B., et al. (1987) Nucleic Acides Res. 15 (13) :5181-97.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_006588.1

Uniprot Accession Number

P11142

Species

Human

AA Sequence

MSKGPAVGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA MNPTNTVFDA KRLIGRRFDD AVVQSDMKHW PFMVVNDAGR PKVQVEYKGE TKSFYPEEVS SMVLTKMKEI AEAYLGKTVT NAVVTVPAYF NDSQRQATKD AGTIAGLNVL RIINEPTAAA IAYGLDKKVG AERNVLIFDL GGGTFDVSIL TIEDGIFEVK STAGDTHLGG EDFDNRMVNH FIAEFKRKHK KDISENKRAV RRLRTACERA KRTLSSSTQA SIEIDSLYEG IDFYTSITRA RFEELNADLF RGTLDPVEKA LRDAKLDKSQ IHDIVLVGGS TRIPKIQKLL QDFFNGKELN KSINPDEAVA YGAAVQAAIL SGDKSENVQD LLLLDVTPLS LGIETAGGVM TVLIKRNTTI PTKQTQTFTT YSDNQPGVLI QVYEGERAMT KDNNLLGKFE LTGIPPAPRG VPQIEVTFDI DANGILNVSA VDKSTGKENK ITITNDKGRL SKEDIERMVQ EAEKYKAEDE KQRDKVSSKN SLESYAFNMK ATVEDEKLQG KINDEDKQKI LDKCNEIINW LDKNQTAEKE EFEHQQKELE KVCNPIITKL YQSAGGMPGG MPGGFPGGGA PPSGGASSGP TIEEVD