Recombinant human Galectin-3/LGALS3 protein

Galectin-3 is a member of the family of animal lectins, which selectively binds beta-galactoside residues. This protein is secreted from cells by ectocytosis, which is independent of the classical secretory pathway through the endoplasmic reticulum/Golgi network. Galectin-3 has been associated with the inhibition of apoptosis and the progression of cancer. It is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. Recombinant Galectin-3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

The ED50 for this effect is less or equal to 15 µg/mL. Measured by its ability to agglutinate human red blood cells.

Product Name Alternative

Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, MAC2, Advanced glycation end-product receptor 3, GALIG

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

The ED50 for this effect is less or equal to 15 µg/mL. Measured by its ability to agglutinate human red blood cells.

Molecular Weight

28.3 kDa (270aa) confirmed by MALDI-TOF

Additionnal Information

Galectin3, CBP35, GAL3, GALBP, GALIG, LGALS2, MAC2., 35 kDa lectin, Carbohydrate binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, Galectin 3 internal gene, included, Galectin-3, Galectin3, GBP, IgE binding protein, IgE-binding protein, L 31, L 34, L-34 galactoside-binding lectin, L31, LGALS3, Laminin-binding protein, Lectin L-29, MAC 2 antigen, Lectin, galactose binding, soluble 3, Mac-2, Macrophage galactose-specific lectin, MGC105387., ATGP0414-10 µg, ATGP0414-20 µg, ATGP0414-50 µg, ATGP0414-100 µg, ATGP0414-250 µg, ATGP0414-500 µg, ATGP0414-1 mg, ATGP0414-10, ATGP0414-20, ATGP0414-50, ATGP0414-100, ATGP0414-250, ATGP0414-500, ATGP0414-1

References & Citations

Barondes SH., et al. (1994) J Biol Chem. 269 (33) :20807-10. ; ; Kadrofske MM., et al. (1998) Arch Biochem Biophys. 349 (1) :7-20.

Other References

Campanero-Rhodes M.A et al., Exploration of Galectin Ligands Displayed on Gram-Negative Respiratory Bacterial Pathogens with Different Cell Surface Architectures. (Biomolecules. 2021) {https://pubmed.ncbi.nlm.nih.gov/33919637/}; ; Fabregat FN et al., Structure of the Lipopolysaccharide from Paenalcaligenes hominis: A Chemical Perspective on Immune Recognition. (JACS Au. 2025) {https://doi.org/10.1021/jacsau.5c00441}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl

Scientific Category

Differentiation & Regeneration

NCBI Accession Number

NP_002297.2

Uniprot Accession Number

P17931

Species

Human

AA Sequence

MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI