Recombinant mouse Glutathione S-transferase mu 1/GSTM1 protein

GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur) . The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 40,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Product Name Alternative

PmGT10, GSTM1, GST1, GST mu, GST class mu 1, GST class Mu, GST 1-1, Glutathione S-transferase Mu 1, Glutathione S transferase mu

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 40,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Molecular Weight

28.1 kDa (238aa) confirmed by MALDI-TOF

Additionnal Information

PmGT10, GSTM-1, GSTM1, GSTM 1, GST-1, GST1, GST mu, GST class-mu1, GST class-mu 1, GST class mu1, GST class mu 1, GST class mu, GST 1-1, GST 1 1, GST 1, Glutathione S-Transferase Mu1, Glutathione S-transferase Mu 1, Glutathione S Transferase Mu 1, Glutathione S transferase mu, ATGP0413-10 µg, ATGP0413-20 µg, ATGP0413-50 µg, ATGP0413-100 µg, ATGP0413-250 µg, ATGP0413-500 µg, ATGP0413-1 mg, ATGP0413-010, ATGP0413-020, ATGP0413-050, ATGP0413-100, ATGP0413-250, ATGP0413-500, ATGP0413-01M

References & Citations

Goekkurt E., et al. (2009) J Clin Oncol. 27 (17) :2863-73. ; ; Koutros S., et al. (2009) Cancer Res. 69 (5) :1877-84.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_034488

Uniprot Accession Number

P10649

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK