Recombinant human LIN-28A protein

Lin-28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. It is marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. It has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Recombinant Lin28 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Zinc finger CCHC domain-containing protein 1, ZCCHC1, Tex17, RNA binding protein lin 28, LIN28A, LIN-28, Lin 28 homolog A, Lin 28 homolog, FLJ12457, CSDD1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

21.1 kDa (191aa) confirmed by MALDI-TOF

Additionnal Information

Zinc finger CCHC domain-containing protein 1 AL024421.Lin 28, Zinc finger CCHC domain-containing protein 1, Zinc finger CCHC domain containing protein 1, ZCCHC1, Tex17, RNA binding protein lin 28, LIN28A, LIN-28A, LIN-28, LIN28, LIN 28, LIN 28A, LIN 28 A, Lin 28 homolog A, Lin 28 homolog, FLJ12457, CSDD1, "Lin 28 homolog (C, elegans) ", ATGP0412-10 µg, ATGP0412-20 µg, ATGP0412-50 µg, ATGP0412-100 µg, ATGP0412-250 µg, ATGP0412-500 µg, ATGP0412-1 mg, ATGP0412-010, ATGP0412-020, ATGP0412-050, ATGP0412-100, ATGP0412-250, ATGP0412-500, ATGP0412-01M

References & Citations

Moss E.G., et al. (2003) Dev. Biol. 258:432-442; ; Heo I., et al. (2009) Cell. 138:696-708.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 0.1mM PMSF

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_078950

Uniprot Accession Number

Q9H9Z2

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH RSMGICKWFN VRMGFGFLSM TARAGVALDP PVDVFVHQSK LHMEGFRSLK EGEAVEFTFK KSAKGLESIR VTGPGGVFCI GSERRPKGKS MQKRRSKGDR CYNCGGLDHH AKECKLPPQP KKCHFCQSIS HMVASCPLKA QQGPSAQGKP TYFREEEEEI HSPTLLPEAQ N