Recombinant human Galectin-4/LGALS4 protein

Galectin-4 belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions, which inhibits chronic inflammations, GVHD, and allergic reactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. Recombinant Galectin-4 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

The ED50 range ≤2 µg/mL. Measured by its ability to ability to agglutinate human red blood cells.

Product Name Alternative

Gal-4, Antigen NY-CO-27, L-36 lactose-binding protein, L36LBP, Lactose-binding lectin 4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

The ED50 ≤2 µg/mL. Measured by its ability to ability to agglutinate human red blood cells.

Molecular Weight

38.1 kDa (343aa) confirmed by MALDI-TOF

Additionnal Information

Galectin4, ATGP0409-10 µg, ATGP0409-20 µg, ATGP0409-50 µg, ATGP0409-100 µg, ATGP0409-250 µg, ATGP0409-500 µg, ATGP0409-1 mg, ATGP0409-10, ATGP0409-20, ATGP0409-50, ATGP0409-100, ATGP0409-250, ATGP0409-500, ATGP0409-1

References & Citations

Huflejt ME., et al. (1997) J Biol Chem. 272 (22) :14294-303. ; ; Huflejt ME., et al. (2004) Glycoconj J. 20 (4) :247-55.;

Other References

Campanero-Rhodes M.A et al., Exploration of Galectin Ligands Displayed on Gram-Negative Respiratory Bacterial Pathogens with Different Cell Surface Architectures. (Biomolecules. 2021) {https://pubmed.ncbi.nlm.nih.gov/33919637/}; ; Fabregat FN et al., Structure of the Lipopolysaccharide from Paenalcaligenes hominis: A Chemical Perspective on Immune Recognition. (JACS Au. 2025) {https://pubmed.ncbi.nlm.nih.gov/33919637/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Adhesion & Movement

NCBI Accession Number

NP_006140

Uniprot Accession Number

P56470

Species

Human

AA Sequence

MAYVPAPGYQ PTYNPTLPYY QPIPGGLNVG MSVYIQGVAS EHMKRFFVNF VVGQDPGSDV AFHFNPRFDG WDKVVFNTLQ GGKWGSEERK RSMPFKKGAA FELVFIVLAE HYKVVVNGNP FYEYGHRLPL QMVTHLQVDG DLQLQSINFI GGQPLRPQGP PMMPPYPGPG HCHQQLNSLP TMEGPPTFNP PVPYFGRLQG GLTARRTIII KGYVPPTGKS FAINFKVGSS GDIALHINPR MGNGTVVRNS LLNGSWGSEE KKITHNPFGP GQFFDLSIRC GLDRFKVYAN GQHLFDFAHR LSAFQRVDTL EIQGDVTLSY VQI