Recombinant human FBPase 1 protein
Fructose-1, 6-bisphosphatase 1 (FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
39.0 kDa (358aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Rabenhorst u., et al. (2009) Hepatology. 50 (4) :1121-9. ; ; Hirota K., et al. (2008) Nature. 456 (7218) :130-4.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_000498.2
Uniprot Accession Number
P09467
Species
Human
AA Sequence
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