Recombinant human FBPase 1 protein

Fructose-1, 6-bisphosphatase 1 (FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

FBPase1, FBP, FBP1, D fructose 1 6 bisphosphate 1 phosphohydrolase 1, EC 3.1.3.11, FBP 1, FBPase 1, Fructose 1 6 bisphosphatase 1 Fructose bisphosphatase 1, Growth inhibiting protein 17, Liver fructose bisphosphatase.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

39.0 kDa (358aa) confirmed by MALDI-TOF

Additionnal Information

FBPase1, FBPase1, FBP, FBP1, D fructose 1 6 bisphosphate 1 phosphohydrolase 1, EC 3.1.3.11, FBP 1, FBPase 1, Fructose 1 6 bisphosphatase 1 Fructose bisphosphatase 1, Growth inhibiting protein 17, Liver fructose bisphosphatase., ATGP0408-10 µg, ATGP0408-20 µg, ATGP0408-50 µg, ATGP0408-100 µg, ATGP0408-250 µg, ATGP0408-500 µg, ATGP0408-1 mg, ATGP0408-10, ATGP0408-20, ATGP0408-50, ATGP0408-100, ATGP0408-250, ATGP0408-500, ATGP0408-1

References & Citations

Rabenhorst u., et al. (2009) Hepatology. 50 (4) :1121-9. ; ; Hirota K., et al. (2008) Nature. 456 (7218) :130-4.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_000498.2

Uniprot Accession Number

P09467

Species

Human

AA Sequence

MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNL VAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ