Recombinant human Aldehyde Dehydrogenase 1-A1/ ALDH1A1 protein

Aldehyde dehydrogenase 1A1 (ALDH1A1), also known as retinal dehydrogenase 1, is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea. (Retinal + NAD+ + H2O = retinoate + NADH) Recombinant ALDH1A1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Aldehyde dehydrogenase 1A1, ALDC, ALDH1, PUMB1, RALDH 1, 3-deoxyglucosone dehydrogenase 1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic 1, Retinal dehydrogenase 1

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

54.8 kDa (501aa) confirmed by MALDI-TOF

Additionnal Information

Aldehyde Dehydrogenase1-A1, ALDH1A1, Retinal dehydrogenase 1, RALDH1, ALDC, ALDH-E1, ALDH1, ALDH11, PuMB1, RALDH1, Acetaldehyde dehydrogenase 1, AHD2, Aldehyde Dehydrogenase, MGC2318, RALDH 1, RalDH1 Aldehyde dehydrogenase 1 family member A1, Aldehyde dehydrogenase 1 soluble, Aldehyde dehydrogenase 1A1, Aldehyde dehydrogenase cytosolic, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase liver cytosolic, ALDH 1, ALDH 1A1, ALDH class 1, ALDH-E1, ALDH1 A1, ATGP0405-10 µg, ATGP0405-20 µg, ATGP0405-50 µg, ATGP0405-100 µg, ATGP0405-250 µg, ATGP0405-500 µg, ATGP0405-1 mg, ATGP0405-10, ATGP0405-20, ATGP0405-50, ATGP0405-100, ATGP0405-250, ATGP0405-500, ATGP0405-1

References & Citations

Pereira F., et al. (1991) Biochem Biophys Res Commun. 175 (3) :831-8.

Other References

Stiti N, et al. Nicotinamide Cofactors Suppress Active-Site Labeling of Aldehyde Dehydrogenases. (ACS Chem Biol. 2016) {https://pubmed.ncbi.nlm.nih.gov/26990764/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_000680.2

Uniprot Accession Number

P00352

Species

Human

AA Sequence

MSSSGTPDLP VLLTDLKIQY TKIFINNEWH DSVSGKKFPV FNPATEEELC QVEEGDKEDV DKAVKAARQA FQIGSPWRTM DASERGRLLY KLADLIERDR LLLATMESMN GGKLYSNAYL NDLAGCIKTL RYCAGWADKI QGRTIPIDGN FFTYTRHEPI GVCGQIIPWN FPLVMLIWKI GPALSCGNTV VVKPAEQTPL TALHVASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDID KVAFTGSTEV GKLIKEAAGK SNLKRVTLEL GGKSPCIVLA DADLDNAVEF AHHGVFYHQG QCCIAASRIF VEESIYDEFV RRSVERAKKY ILGNPLTPGV TQGPQIDKEQ YDKILDLIES GKKEGAKLEC GGGPWGNKGY FVQPTVFSNV TDEMRIAKEE IFGPVQQIMK FKSLDDVIKR ANNTFYGLSA GVFTKDIDKA ITISSALQAG TVWVNCYGVV SAQCPFGGFK MSGNGRELGE YGFHEYTEVK TVTVKISQKN S