Recombinant human Enolase 1/ENO1 protein
Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
47.1 kDa (434aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Das R., et al. (2009) Blood. 113 (22) :5371-2.; ; ueno NT., et al. (2008) Cancer Res. 68 (22) :9302-10.;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM MgSO4, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_001419.1
Uniprot Accession Number
P06733
Species
Human
AA Sequence
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