Recombinant human RAC1 protein

Rac1, belonging to the Rho small GTPase family, regulates the actin cytoskeleton but also other cellular processes. RAC1 have been shown to be involved in the regulation of cell-cell adhesion. Recombinant human RAC1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related C3 botulinum toxin substrate 1 isoform, MIG5, p21-Rac1, TC-25, Ras-related C3 botulinum toxin substrate 1 isoform Migration inducing gene 5, Migration inducing protein 5, p21 Rac1, Rac 1, Ras like protein TC25, TC25.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

23.6 kDa (212aa) confirmed by MALDI-TOF

Additionnal Information

RAC1, Ras-related C3 botulinum toxin substrate 1 isoform, MIG5, p21-Rac1, TC-25, Ras-related C3 botulinum toxin substrate 1 isoform Migration inducing gene 5, Migration inducing protein 5, p21 Rac1, Rac 1, Ras like protein TC25, TC25., ATGP0403-10 µg, ATGP0403-20 µg, ATGP0403-50 µg, ATGP0403-100 µg, ATGP0403-250 µg, ATGP0403-500 µg, ATGP0403-1 mg, ATGP0403-10, ATGP0403-20, ATGP0403-50, ATGP0403-100, ATGP0403-250, ATGP0403-500, ATGP0403-1

References & Citations

Chrostek A., et al. (2006) Mol Cell Biol. 26: 6957-70.; ; Sun CX., et al. (2004) Blood. 104 (12) :3758-65.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 1mM EDTA, 10% glycerol

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_008839

Uniprot Accession Number

P63000

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKRKRKCL LL