Recombinant S. Japonicum Glutathione S-transferase/GST protein

Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur) . The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E. coli source. Recombinant Schistosoma japonicum GST, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 10 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Product Name Alternative

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

Expression System

E.coli

Antigen Species

Schistosoma japonicum

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 10unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Molecular Weight

28.3 kDa (244aa) confirmed by MALDI-TOF

Additionnal Information

Glutathione S-Transferase, GST, Glutathione S-Transferase class-mu 26 kDa isozyme, Sj26 antigen, SjGST, Glutathione S-Transferase class-mu 26 kDa isozyme Glutathione S Transferase., ATGP0401-10 µg, ATGP0401-20 µg, ATGP0401-50 µg, ATGP0401-100 µg, ATGP0401-250 µg, ATGP0401-500 µg, ATGP0401-1 mg, ATGP0401-10, ATGP0401-20, ATGP0401-50, ATGP0401-100, ATGP0401-250, ATGP0401-500, ATGP0401-1

References & Citations

Hayes JD., et al. (1995) . Crit Rev Biochem Mol Biol. 30 (6) :445-600; ; Bekett GJ., et al. (1987) . J. Clin Biochem Nutr. 2:1-24; ; Smith DB., et al. (1988) . Mol Biochem Parasitol. 27 (2-3) :249-56.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AAB59203.1

Uniprot Accession Number

P08515

Species

Schistosoma japonicum

AA Sequence

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPK