Recombinant human VPS28 protein

VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

VPS28, Vacuolar Protein Sorting 28 acuolar protein sorting 28, Vacuolar Protein Sorting 28, H-Vps28, ESCRT-I complex subunit VPS28

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

25.4 kDa (221aa) confirmed by MALDI-TOF

Additionnal Information

VPS28, Vacuolar protein sorting 28 homolog, Vacuolar Protein Sorting 28 acuolar protein sorting 28, Vacuolar protein sorting 28 (yeast), Vacuolar Protein Sorting 28, H-Vps28, ESCRT-I complex subunit VPS28, ATGP0400-10 µg, ATGP0400-20 µg, ATGP0400-50 µg, ATGP0400-100 µg, ATGP0400-250 µg, ATGP0400-500 µg, ATGP0400-1 mg, ATGP0400-010, ATGP0400-020, ATGP0400-050, ATGP0400-100, ATGP0400-250, ATGP0400-500, ATGP0400-01M

References & Citations

Woodman P., et al. (2001) J Biol Chem. 276 (15) :11735-42.; ; Bieniasz PD., et al. (2003) J Virol. 77 (8) :4794-804.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol

Scientific Category

Ubiquitination

NCBI Accession Number

NP_057292

Uniprot Accession Number

Q9UK41

Species

Human

AA Sequence

MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A