Recombinant human PRL-3/PTP4A3 protein
Protein tyrosine phosphatase type IVA 3 (PRL-3), as known as PTP4A3, belongs to a small class of prenylated protein tyrosine phosphatases (PTPs) that remove phosphate modifications from tyrosine residues on proteins. This protein enhances cell proliferation, cell motility and invasive activity. High levels of PRL-3 expression are associated with tumorigenesis and metastasis, thus it is overexpressed in metastatic colorectal, ovarian, liver and skin cancers. Recombinant human PRL-3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
21.6 kDa (193aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ooki A., et al. (2009) . Oncol Rep. 21 (6) :1467-75; ; Wang Z., et al. (2009) . Ann Surg Oncol. 16 (1) :208-19.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM EDTA, 1mM DTT, 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_116000
Uniprot Accession Number
O75365
Species
Human
AA Sequence
Frequently Asked Questions
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