Recombinant human PRL-3/PTP4A3 protein

Protein tyrosine phosphatase type IVA 3 (PRL-3), as known as PTP4A3, belongs to a small class of prenylated protein tyrosine phosphatases (PTPs) that remove phosphate modifications from tyrosine residues on proteins. This protein enhances cell proliferation, cell motility and invasive activity. High levels of PRL-3 expression are associated with tumorigenesis and metastasis, thus it is overexpressed in metastatic colorectal, ovarian, liver and skin cancers. Recombinant human PRL-3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Protein tyrosine phosphatase type IVA 3, PTP4A3, PRL3, Protein tyrosine phosphatase type IVA 3 Potentially prenylated protein tyrosine phosphatase, PRL 3, PRL R, PRLR, Protein tyrosine phosphatase 4a3, PTP 4A3, Protein Tyrosine Phosphatase Type IVA Member 3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

21.6 kDa (193aa) confirmed by MALDI-TOF

Additionnal Information

PRL3, PTP4A3, Protein tyrosine phosphatase type IVA 3, PTP4A3, PRL3, Protein tyrosine phosphatase type IVA 3 Potentially prenylated protein tyrosine phosphatase, PRL 3, PRL R, PRLR, Protein tyrosine phosphatase 4a3, PTP 4A3, Protein Tyrosine Phosphatase Type IVA Member 3, ATGP0398-10 µg, ATGP0398-20 µg, ATGP0398-50 µg, ATGP0398-100 µg, ATGP0398-250 µg, ATGP0398-500 µg, ATGP0398-1 mg, ATGP0398-10, ATGP0398-20, ATGP0398-50, ATGP0398-100, ATGP0398-250, ATGP0398-500, ATGP0398-1

References & Citations

Ooki A., et al. (2009) . Oncol Rep. 21 (6) :1467-75; ; Wang Z., et al. (2009) . Ann Surg Oncol. 16 (1) :208-19.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM EDTA, 1mM DTT, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_116000

Uniprot Accession Number

O75365

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MARMNRPAPV EVSYKHMRFL ITHNPTNATL STFIEDLKKY GATTVVRVCE VTYDKTPLEK DGITVVDWPF DDGAPPPGKV VEDWLSLVKA KFCEAPGSCV AVHCVAGLGR APVLVALALI ESGMKYEDAI QFIRQKRRGA INSKQLTYLE KYRPKQRLRF KDPHTHKTRC CVM