Recombinant human TC21/RRAS2 protein
RAS viral (r-ras) oncogene homolog 2 isoform a, also known as RRAS2, belongs to the Ras like GTPase family that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. It is implicated in the pathogenesis of human cancers. Thus, overexpression of active RRAS2 in EpH4 cells induces tumorigenicity through the phosphoinositide 3-kinase, p38 MAPK, and mTOR pathways, and these cells lose their sensitivity to the normal growth inhibitory role of TGF-beta. Recombinant human RRAS2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ras-related protein R-Ras2, Related RAS viral (r-ras) oncogene homolog 2, Ras-like protein TC21, Teratocarcinoma oncogene, TC21
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
25.2 kDa (221aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Erdogan M., et al. (2007) J Biol Chem. 282 (38) :27713-20. ; ; Movilla N., et al. (1999) Oncogene. 18 (43) :5860-9. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA
Scientific Category
Cancer
NCBI Accession Number
NP_036382
Uniprot Accession Number
P62070
Species
Human
AA Sequence
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