Recombinant human CDC42 protein

Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs. Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cell division cycle 42 isoform 1, G25K, Cell division cycle 42 isoform 1 CDC42Hs, Cell division cycle 42, Small GTP binding protein CDC42.

Expression System

E.coli

Antigen Species

Human

Tag

T7-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.4 kDa (203aa) confirmed by MALDI-TOF

Additionnal Information

CDC42, ATGP0395-10 µg, ATGP0395-20 µg, ATGP0395-50 µg, ATGP0395-100 µg, ATGP0395-250 µg, ATGP0395-500 µg, ATGP0395-1 mg, ATGP0395-10, ATGP0395-20, ATGP0395-50, ATGP0395-100, ATGP0395-250, ATGP0395-500, ATGP0395-1

References & Citations

Kathryn P., et al. (2008) J Cell Biol. 183 (6) :1129-1143. ; ; Li R., et al. (1997) J Biol Chem. 272 (52) :32830-5. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001782

Uniprot Accession Number

P60953

Species

Human

AA Sequence

MQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC