Recombinant human CDC42 protein
Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs. Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cell division cycle 42 isoform 1, G25K, Cell division cycle 42 isoform 1 CDC42Hs, Cell division cycle 42, Small GTP binding protein CDC42.
Expression System
E.coli
Antigen Species
Human
Tag
T7-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
22.4 kDa (203aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kathryn P., et al. (2008) J Cell Biol. 183 (6) :1129-1143. ; ; Li R., et al. (1997) J Biol Chem. 272 (52) :32830-5. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001782
Uniprot Accession Number
P60953
Species
Human
AA Sequence
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