Recombinant mouse VEGF 120/VEGFA protein
VEGF-A, also known as vascular endothelial growth factor, belong to the VEGF family that mediate angiogenesis. This protein is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis. Recombinant VEGF-A protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse VEGF in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤ 15 ng/mL.
Product Name Alternative
Vascular endothelial growth factor A isoform 3, Vegf-a, Vegf120, Vpf
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤ 15 ng/mL.
Molecular Weight
16.3 kDa (141aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gerber H., et al. (2002) Nature. 417: 954-958.; ; Shibuya M., et al. (2006) J Biochem Mol Biol. 39:469-478.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Growth factors
NCBI Accession Number
NP_001020428.1
Uniprot Accession Number
Q00731-3
Species
Mouse
AA Sequence
APTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R
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