Recombinant mouse VEGF 120/VEGFA protein

VEGF-A, also known as vascular endothelial growth factor, belong to the VEGF family that mediate angiogenesis. This protein is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis. Recombinant VEGF-A protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse VEGF in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤ 15 ng/mL.

Product Name Alternative

Vascular endothelial growth factor A isoform 3, Vegf-a, Vegf120, Vpf

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤ 15 ng/mL.

Molecular Weight

16.3 kDa (141aa) confirmed by MALDI-TOF

Additionnal Information

Vascular endothelial growth factor A isoform 3, Vegf-a, Vegf120, Vpf, ATGP0389-10 µg, ATGP0389-20 µg, ATGP0389-50 µg, ATGP0389-100 µg, ATGP0389-250 µg, ATGP0389-500 µg, ATGP0389-1 mg, ATGP0389-10, ATGP0389-20, ATGP0389-50, ATGP0389-100, ATGP0389-250, ATGP0389-500, ATGP0389-1