Recombinant human Visinin-like protein-1/VILIP protein

Visinin-like protein-1, also known as VILIP-1, belongs to a family of neuronal calcium sensor proteins. VILIP-1 is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Also, it is expressed in pancreatic beta-cells. VILIP-1 elevation enhances insulin secretion in cAMP-associated manner. Down-regulation of VILIP-1 decreased cAMP accumulation but increased insulin gene transcription. Recombinant human Visinin-like protein-1 was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

VILIP, VILIP-1, VLP-1, HLP3, VSNL1, Hippocalcin like protein 3, HLP 3, HPCAL 3, HPCAL3, HuVISL1, Neurocalcin alpha, NVL 1, NVL1, VILIP, Visinin.Visinin like 1, Visinin like protein 1.VISL 1.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.1 kDa (191aa) confirmed by MALDI-TOF

Additionnal Information

Visinin-like protein1, VILIP, VILIP-1, VLP-1, HLP3, VSNL1, Hippocalcin like protein 3, HLP 3, HPCAL 3, HPCAL3, HuVISL1, Neurocalcin alpha, NVL 1, NVL1, VILIP, Visinin.Visinin like 1, Visinin like protein 1.VISL 1., ATGP0384-10 µg, ATGP0384-20 µg, ATGP0384-50 µg, ATGP0384-100 µg, ATGP0384-250 µg, ATGP0384-500 µg, ATGP0384-1 mg, ATGP0384-10, ATGP0384-20, ATGP0384-50, ATGP0384-100, ATGP0384-250, ATGP0384-500, ATGP0384-1

References & Citations

Lenz SE., et al. (1996) Biochem Biophys Res Commun. 225 (3) :1078-83.; ; Braunewell KH., et al. (1997) J Neurochem. 68 (5) :2129-39.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_003376

Uniprot Accession Number

P62760

Species

Human

AA Sequence

MGKQNSKLAP EVMEDLVKST EFNEHELKQW YKGFLKDCPS GRLNLEEFQQ LYVKFFPYGD ASKFAQHAFR TFDKNGDGTI DFREFICALS ITSRGSFEQK LNWAFNMYDL DGDGKITRVE MLEIIEAIYK MVGTVIMMKM NEDGLTPEQR VDKIFSKMDK NKDDQITLDE FKEAAKSDPS IVLLLQCDIQ K