Recombinant human RKIP/PEBP1 protein

Phosphatidylethanolamine binding protein 1 (PEBP1), also known as Raf kinase inhibitor protein (RKIP), is a member of the phosphatidylethanolamine-binding protein family and a serine protease inhibitor which inhibits thrombin, neuropsin. PEBP1 plays a pivotal modulatory role in several protein kinase signaling cascades. Protein kinase C (PKC) phosphorylates PEBP1, resulting in release of Raf-1 and activation of MEK and ERK. Expressed in many tissues, PEBP1 is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression. Recombinant human PEBP1 was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phosphatidylethanolamine binding protein 1, PBP, Prostatic binding protein, RKIP, HCNP, HCNPpp, PEBP, Raf kinase inhibitory protein, Hippocampal cholinergic neurostimulating peptide

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

21 kDa (187aa) confirmed by MALDI-TOF

Additionnal Information

RKIP, PEBP1, Phosphatidylethanolamine binding protein 1, HCNP, PBP, PEBP, Phosphatidylethanolamine binding protein 1 Hippocampal cholinergic neurostimulating peptide, Neuropolypeptide h3, HCNPpp, PEBP, PEBP 1, PEBP1, Phosphatidylethanolamine binding protein, Prostatic binding protein, R kip, Raf kinase inhibitory protein RKIP., ATGP0382-10 µg, ATGP0382-20 µg, ATGP0382-50 µg, ATGP0382-100 µg, ATGP0382-250 µg, ATGP0382-500 µg, ATGP0382-1 mg, ATGP0382-10, ATGP0382-20, ATGP0382-50, ATGP0382-100, ATGP0382-250, ATGP0382-500, ATGP0382-1

References & Citations

Keller ET., et al. (2004) Biochem Pharmacol. 68 (6) :1049-53. ; ; Park S., et al. (2005) Oncogene. 24 (21) :3535-40. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_002558

Uniprot Accession Number

P30086

Species

Human

AA Sequence

MPVDLSKWSG PLSLQEVDEQ PQHPLHVTYA GAAVDELGKV LTPTQVKNRP TSISWDGLDS GKLYTLVLTD PDAPSRKDPK YREWHHFLVV NMKGNDISSG TVLSDYVGSG PPKGTGLHRY VWLVYEQDRP LKCDEPILSN RSGDHRGKFK VASFRKKYEL RAPVAGTCYQ AEWDDYVPKL YEQLSGK