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Recombinant human 4E-BP1/EIF4EBP1 protein

EIF4E-BP1, also known as eukaryotic translation initiation factor 4E-binding protein 1, is a member of a family of translation repressor proteins. This protein regulates eukaryotic translation initiation factor 4E (eIF4E) activity by preventing its assembly into the eIF4F complex and mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. Recombinant EIF4EBP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, MGC4316, Eukaryotic translation initiation factor 4E binding protein 1, EIF4EBP1, EIF4E-binding protein 1, BP-1, 4EBP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BCA assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

14.7 kDa (138aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, MGC4316, Eukaryotic translation initiation factor 4E-binding protein 1, Eukaryotic translation initiation factor 4E binding protein 1, EIF4EBP1, EIF4E-binding protein 1, BP-1, 4E-BP1, 4EBP1, ATGP0379-10 µg, ATGP0379-20 µg, ATGP0379-50 µg, ATGP0379-100 µg, ATGP0379-250 µg, ATGP0379-500 µg, ATGP0379-1 mg, ATGP0379-010, ATGP0379-020, ATGP0379-050, ATGP0379-100, ATGP0379-250, ATGP0379-500, ATGP0379-01M

References & Citations

Pause A., et al. (1994) Nature. 371:762-767.; ; Olsen JV., et al. (2006) Cell. 127 (3) :635-48.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004086.1

Uniprot Accession Number

Q13541

Species

Human

AA Sequence

MSGGSSCSQT PSRAIPATRR VVLGDGVQLP PGDYSTTPGG TLFSTTPGGT RIIYDRKFLM ECRNSPVTKT PPRDLPTIPG VTSPSSDEPP MEASQSHLRN SPEDKRAGGE ESQFEMDI

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