Recombinant human Biliverdin Reductase A/BLVRA protein

BLVRA, also known as biliverdin reductase A, belongs to the gfo/idh/mocA family. This protein is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. (Bilirubin + NAD (P) + = biliverdin + NAD (P) H) Recombinant BLVRA protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 450 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of biliverdin of NADPH per minute at pH 8.8 at 25C.

Product Name Alternative

Biliverdin reductase A, BLVR, BVR, BVRA, BLVRA, Biliverdin reductase A Biliverdin IX alpha reductase, BLVR A, BVR A, Zinc metalloprotein.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

Enzyme Activity, SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 450pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of biliverdin of NADPH per minute at pH 8.8 at 25C.

Molecular Weight

33.3 kDa (295aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Biliverdin Reductase A, BLVRA, Biliverdin reductase A, BLVR, BVR, BVRA, BLVRA, Biliverdin reductase A Biliverdin IX alpha reductase, BLVR A, BVR A, Zinc metalloprotein., ATGP0377-10 µg, ATGP0377-20 µg, ATGP0377-50 µg, ATGP0377-100 µg, ATGP0377-250 µg, ATGP0377-500 µg, ATGP0377-1 mg, ATGP0377-10, ATGP0377-20, ATGP0377-50, ATGP0377-100, ATGP0377-250, ATGP0377-500, ATGP0377-1

References & Citations

Kravets A., et al. (2004) J Biol Chem. 279 (19) :19916-23.; ; Franklin E., et al. (2007) Biochem J. 405 (1) :61-7.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_000703

Uniprot Accession Number

P53004

Species

Human

AA Sequence

MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK