Recombinant human Profilin 1/PFN1 protein

Profilin1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein significantly enhances skin wound healing in-vitro and in-vivo that may be mediated by purinergic receptors. It is also active in endothelial cell migration and vessel sprouting. It is thought to regulate actin polymerization in response to extracellular signals. Recombinant Profilin1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Profilin-1, Profilin I, Profilin, Pfn1, Actin binding protein

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

15 kDa (140aa) confirmed by MALDI-TOF

Additionnal Information

Profilin-I, ProfilinI, Profilin-1, Profilin1, Profilin I, Profilin 1, PFN-1, PFN1, PFN 1, Pfn, Actin binding protein, ATGP0376-10 µg, ATGP0376-20 µg, ATGP0376-50 µg, ATGP0376-100 µg, ATGP0376-250 µg, ATGP0376-500 µg, ATGP0376-1 mg, ATGP0376-010, ATGP0376-020, ATGP0376-050, ATGP0376-100, ATGP0376-250, ATGP0376-500, ATGP0376-01M

References & Citations

Kwiatkowski DJ, et al. (1988) J. Biol. Chem. 263 (12) : 5910-5; ; Harbeck B., et al. (2000) J. Biol. Chem. 275 (40) : 30817-25.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_005013

Uniprot Accession Number

P07737

Species

Human

AA Sequence

MAGWNAYIDN LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VNITPAEVGV LVGKDRSSFY VNGLTLGGQK CSVIRDSLLQ DGEFSMDLRT KSTGGAPTFN VTVTKTDKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY