Recombinant human OAS1 protein

OAS1 is an enzyme included in the 2', 5'-oligoadenylate synthase family. This enzyme is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2', 5'-oligoadenylates (2-5As) . These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. OAS1 may play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis. Recombinant OAS1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

2'5'-oligoadenylate synthetase 1 isoform2, IFI-4, OIAS, OIASI, 2',5'-oligoadenylate synthetase 1, isoform2 (2 5') oligo (A) synthetase 1, 2' 5' oligo A synthetase 1, 2 5 Oligoadenylate Synthetase 1, 2 5A synthetase 1, 2' 5' oligoadenylate synthetase 1, 2' 5' oligoisoadenylate synthetase 1, 2'5' oligoadenylate synthetase 1, 2'5' oligoisoadenylate synthetase 1, p46/p42 OAS, 2'5' oligo A synthetase 1, E18/E16, IFI 4, IFI4, OAS 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

43.9 kDa (384aa) confirmed by MALDI-TOF

Additionnal Information

OAS1, 2'5'-oligoadenylate synthetase 1 isoform2, IFI-4, OIAS, OIASI, 2',5'-oligoadenylate synthetase 1, isoform2 (2 5') oligo (A) synthetase 1, 2' 5' oligo A synthetase 1, 2 5 Oligoadenylate Synthetase 1, 2 5A synthetase 1, 2' 5' oligoadenylate synthetase 1, 2' 5' oligoisoadenylate synthetase 1, 2'5' oligoadenylate synthetase 1, 2'5' oligoisoadenylate synthetase 1, p46/p42 OAS, 2'5' oligo A synthetase 1, E18/E16, IFI 4, IFI4, OAS 1, ATGP0375-10 µg, ATGP0375-20 µg, ATGP0375-50 µg, ATGP0375-100 µg, ATGP0375-250 µg, ATGP0375-500 µg, ATGP0375-1 mg, ATGP0375-10, ATGP0375-20, ATGP0375-50, ATGP0375-100, ATGP0375-250, ATGP0375-500, ATGP0375-1

References & Citations

Rios JJ., et al. (2007) BMC Genomics. 8:313. ; ; Tessier MC., et al. (2006) J Med Genet. 43 (2) :129-32.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002525.2

Uniprot Accession Number

P00973

Species

Human

AA Sequence

MMDLRNTPAK SLDKFIEDYL LPDTCFRMQI NHAIDIICGF LKERCFRGSS YPVCVSKVVK GGSSGKGTTL RGRSDADLVV FLSPLTTFQD QLNRRGEFIQ EIRRQLEACQ RERAFSVKFE VQAPRWGNPR ALSFVLSSLQ LGEGVEFDVL PAFDALGQLT GSYKPNPQIY VKLIEECTDL QKEGEFSTCF TELQRDFLKQ RPTKLKSLIR LVKHWYQNCK KKLGKLPPQY ALELLTVYAW ERGSMKTHFN TAQGFRTVLE LVINYQQLCI YWTKYYDFKN PIIEKYLRRQ LTKPRPVILD PADPTGNLGG GDPKGWRQLA QEAEAWLNYP CFKNWDGSPV SSWILLVRPP ASSLPFIPAP LHEA