Recombinant human E2-EPF/UBE2S protein

UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins. Recombinant uBE2S protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin-conjugating enzyme E2S, E2-EPF, E2EPF, EPF5, Ubiquitin-conjugating enzyme S, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S, Ubiquitin conjugating enzyme E2 24 kD, Ubiquitin conjugating enzyme E2S

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.9 kDa (258aa) confirmed by MALDI-TOF

Additionnal Information

UBE2S, ubiquitin-conjugating enzyme E2S, E2-EPF, E2EPF, EPF5, ubiquitin-conjugating enzyme E2S AA409170, MGC101982, uBE2S, ubiquitin carrier protein S, ubiquitin protein ligase S, ubiquitin conjugating enzyme E2 24 kD, ubiquitin conjugating enzyme E2S, ATGP0372-10 µg, ATGP0372-20 µg, ATGP0372-50 µg, ATGP0372-100 µg, ATGP0372-250 µg, ATGP0372-500 µg, ATGP0372-1 mg, ATGP0372-10, ATGP0372-20, ATGP0372-50, ATGP0372-100, ATGP0372-250, ATGP0372-500, ATGP0372-1

References & Citations

Ye Y., et al. (2009) Nat Rev Mol Cell Biol. 10 (11) :755-64. ; ; Windheim M., et al. (2008) Biochem J. 409 (3) :723-9.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT and 20% glycerol

Scientific Category

Ubiquitination

NCBI Accession Number

NP_055316.2

Uniprot Accession Number

Q16763

Species

Human

AA Sequence

MNSN VENLPPHIIR LVYKEVTTLT ADPPDGIKVF PNEEDLTDLQ VTIEGPEGTP YAGGLFRMKL LLGKDFPASP PKGYFLTKIF HPNVGANGEI CVNVLKRDWT AELGIRHVLL TIKCLLIHPN PESALNEEAG RLLLENYEEY AARARLLTEI HGGAGGPSGR AEAGRALASG TEASSTDPGA PGGPGGAEGP MAKKHAGERD KKLAAKKKTD KKRALRRL