Recombinant human E2-EPF/UBE2S protein
UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins. Recombinant uBE2S protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
27.9 kDa (258aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ye Y., et al. (2009) Nat Rev Mol Cell Biol. 10 (11) :755-64. ; ; Windheim M., et al. (2008) Biochem J. 409 (3) :723-9.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT and 20% glycerol
Scientific Category
Ubiquitination
NCBI Accession Number
NP_055316.2
Uniprot Accession Number
Q16763
Species
Human
AA Sequence
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