Recombinant human Dihydrofolate Reductase/DHFR protein
DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Recombinant Dihydrofolate reductase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Dihydrofolate reductase, EC 1.5.1.3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
23.6 kDa (207aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Loveridge EJ., et al. (2009) Biochemistry. 48 (25) :5922-33. ; ; Hu Y., et al. (2009) J Cell Biol. 185 (1) :87-100.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl 2mM DTT, and 30% glycerol
Scientific Category
Redox Proteins
NCBI Accession Number
NP_000782.1
Uniprot Accession Number
P00374
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
5-(1-Cyclohexen-1-yl)-5-ethyl-6-imino-hydrouracil
JIP1 (MAPK8IP1) (NM_005456) Human Recombinant Protein
![MLD Recombinant Rabbit Monoclonal Antibody [PSH02-54]](https://cdn.genprice.uk/products/992/68dd1c8c061270361ea81c1e/imageurl/ha721830.webp)
MLD Recombinant Rabbit Monoclonal Antibody [PSH02-54]
