Recombinant human Dihydrofolate Reductase/DHFR protein

DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Recombinant Dihydrofolate reductase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

Product Name Alternative

Dihydrofolate reductase, EC 1.5.1.3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 2000pmol/min/ug, and is defined as the amount of enzyme that converts 1.0pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

Molecular Weight

23.6 kDa (207aa) confirmed by MALDI-TOF

Additionnal Information

Dihydrofolate Reductase, DHFR, ATGP0371-10 µg, ATGP0371-20 µg, ATGP0371-50 µg, ATGP0371-100 µg, ATGP0371-250 µg, ATGP0371-500 µg, ATGP0371-1 mg, ATGP0371-10, ATGP0371-20, ATGP0371-50, ATGP0371-100, ATGP0371-250, ATGP0371-500, ATGP0371-1

References & Citations

Loveridge EJ., et al. (2009) Biochemistry. 48 (25) :5922-33. ; ; Hu Y., et al. (2009) J Cell Biol. 185 (1) :87-100.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl 2mM DTT, and 30% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_000782.1

Uniprot Accession Number

P00374

Species

Human

AA Sequence

MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND