Recombinant human CHMP2A protein

CHMP2A, also known as chromatin modifying protein 2A, belongs to the SNF7 family and it is component of the ESCRT III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. Recombinant CHMP2A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Charged multivesicular body protein 2A, Chromatin-modifying protein 2a, CHMP2a, Putative breast adenocarcinoma marker BC-2, Vacuolar protein sorting-associated protein 2-1, Vps2-1, hVps2-1, BC-2, CHMP2, VPS2A, VPS2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.2 kDa (242aa) confirmed by MALDI-TOF

Additionnal Information

CHMP2A, Chromatin modifying protein 2A, BC-2, BC2, CHMP2, VPS2, VPS2A, Chromatin modifying protein 2A Charged multivesicular body protein 2a Vps2 1, hVps2 1, putative breast adenocarcinoma marker, Putative breast adenocarcinoma marker BC2, Vacuolar protein sorting associated protein 2 1., ATGP0370-10 µg, ATGP0370-20 µg, ATGP0370-50 µg, ATGP0370-100 µg, ATGP0370-250 µg, ATGP0370-500 µg, ATGP0370-1 mg, ATGP0370-10, ATGP0370-20, ATGP0370-50, ATGP0370-100, ATGP0370-250, ATGP0370-500, ATGP0370-1

References & Citations

Lata S., et al. (2008) Science. 321 (5894) :1354-7. ; ; Agromayor M., et al. (2006) J Biol Chem. 281 (32) :23083-91.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 30% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_055268.1

Uniprot Accession Number

O43633

Species

Human

AA Sequence

MDLLFGRRKT PEELLRQNQR ALNRAMRELD RERQKLETQE KKIIADIKKM AKQGQMDAVR IMAKDLVRTR RYVRKFVLMR ANIQAVSLKI QTLKSNNSMA QAMKGVTKAM GTMNRQLKLP QIQKIMMEFE RQAEIMDMKE EMMNDAIDDA MGDEEDEEES DAVVSQVLDE LGLSLTDELS NLPSTGGSLS VAAGGKKAEA AASALADADA DLEERLKNLR RD