Recombinant human CCL17/TARC protein
CCL17 is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC) . This protein is involved in immunoregulatory and inflammatory processes. It is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. It specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. CCL17 was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
10.3 kDa (92aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Imai T., et al. (1996) J Biol Chem. 271 (35) : 21514-21.; ; Kakinuma T., et al. (2002) Cytokine. (1) :1-6.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_002978
Uniprot Accession Number
Q92583
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MARGTNVGRE CCLEYFKGAI PLRKLKTWYQ TSEDCSRDAI VFVTVQGRAI CSDPNNKRVK NAVKYLQSLE RS
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