Recombinant human SAT1 protein

Diamine N-acetyltransferase 1 (or Spermidine/spermine-N1-acetyltransferase, SSAT1), as known as SAT-1, is a polyamine catabolic enzyme which catalyzes the acetylation of polyamines. This protein plays an important role in polyamine homoeostasis, since acetylated products are either excreted from the cell or oxidized by acetylpolyamine oxidase. A variety of other effects of increased SAT-1 activity include death of pancreatic cells, blockage of regenerative tissue growth, behavioral changes, keratosis follicularis spinulosa decalvans (KFSD), and hair loss. Recombinant human SAT-1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Spermidine/spermine N1-acetyltransferase 1, DC21, KFSD, KFSDX, SAT, SSAT, SSAT-1, SAT1, Diamine acetyltransferase 1, EC 2.3.1.57, Polyamine N-acetyltransferase 1, Putrescine acetyltransferase, Spermidine/spermine N (1) -acetyltransferase 1, SSAT 1.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.1 kDa (191aa) confirmed by MALDI-TOF

Additionnal Information

Diamine N-acetyltransferase1, spermidine/spermine N1-acetyltransferase 1, DC21, KFSD, KFSDX, SAT, SSAT, SSAT-1, SAT1, Diamine acetyltransferase 1, EC 2.3.1.57, Polyamine N-acetyltransferase 1, Putrescine acetyltransferase, Spermidine/spermine N (1) -acetyltransferase 1, SSAT 1., ATGP0364-10 µg, ATGP0364-20 µg, ATGP0364-50 µg, ATGP0364-100 µg, ATGP0364-250 µg, ATGP0364-500 µg, ATGP0364-1 mg, ATGP0364-10, ATGP0364-20, ATGP0364-50, ATGP0364-100, ATGP0364-250, ATGP0364-500, ATGP0364-1

References & Citations

Pegg AE., et al. (2008) . Am J Physiol Endocrinol Metab. 294: E995-E1010, ; ; Janne J., et al. (2006) . J Cell Mol Med. 10 (4) :933-45.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002961

Uniprot Accession Number

P21673

Species

Human

AA Sequence

MAKFVIRPAT AADCSDILRL IKELAKYEYM EEQVILTEKD LLEDGFGEHP FYHCLVAEVP KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY RGFGIGSEIL KNLSQVAMRC RCSSMHFLVA EWNEPSINFY KRRGASDLSS EEGWRLFKID KEYLLKMATE E