Recombinant human Apolipoprotein A1/APOA1 protein

Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It is the major protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion. And it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the ApoA-1 gene are associated with HDL deficiency including Tangier disease. Recombinant human Apolipoprotein A-1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

APOA1, apolipoprotein A-I, Apolipoprotein A1, Apo-AI, ApoA-I

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

30.3 kDa (264aa) confirmed by MALDI-TOF

Additionnal Information

Apolipoprotein A-I, ApoA1, APOA1, apolipoprotein A-I, Apolipoprotein A1, Apo-AI, ApoA-I, ATGP0363-10 µg, ATGP0363-20 µg, ATGP0363-50 µg, ATGP0363-100 µg, ATGP0363-250 µg, ATGP0363-500 µg, ATGP0363-1 mg, ATGP0363-10, ATGP0363-20, ATGP0363-50, ATGP0363-100, ATGP0363-250, ATGP0363-500, ATGP0363-1

References & Citations

Wang J., et al. (2009) . Int J Biol Sci. 5 (7) :637-46.; ; Khadem-Ansari MH., et al. (2009) . Singapore Med J. 50 (6) :610-3;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_000030

Uniprot Accession Number

P02647

Species

Human

AA Sequence

MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ